Revista Brasileira de Entomologia Revista Brasileira de Entomologia
Rev Bras Entomol 2018;62:19-22 - Vol. 62 Núm.1 DOI: 10.1016/j.rbe.2017.11.007
Biological Control and Crop Protection
Standard method for detecting Bombyx mori nucleopolyhedrovirus disease-resistant silkworm varieties
Qiong Yang, , Dong Xu Xing, Qing Rong Li, Yang Xiao, Ming Qiang Ye
Sericulture & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China
Recebido 24 Março 2017, Aceitaram 30 Novembro 2017

Bombyx mori nucleopolyhedrovirus (BmNPV) disease is one of the most serious silkworm diseases, and it has caused great economic losses to the sericulture industry. So far, the disease has not been controlled effectively by therapeutic agents. Breeding resistant silkworm varieties breeding may be an effective way to improve resistance to BmNPV and reduce economic losses. A precise resistance-detection method will help to accelerate the breeding process. For this purpose, here we described the individual inoculation method (IIM). Details of the IIM include pathogen BmNPV preparation, mulberry leaf size, pathogen volume, rearing conditions, course of infection, and breeding conditions. Finally, a resistance comparison experiment was performed using the IIM and the traditional group inoculation method (GIM). The incidence of BmNPV infection and the within-group variance results showed that the IIM was more precise and reliable than the GIM.

Bombyx mori nucleopolyhedrovirus, Group inoculation method, Individual inoculation method, Resistance detection, Silkworm
Rev Bras Entomol 2018;62:19-22 - Vol. 62 Núm.1 DOI: 10.1016/j.rbe.2017.11.007