Revista Brasileira de Entomologia Revista Brasileira de Entomologia
Rev Bras Entomol 2018;62:119-24 - Vol. 62 Núm.2 DOI: 10.1016/j.rbe.2017.12.004
Systematics, Morphology and Biogeography
Morphometric and molecular differences among Calvertius tuberosus (Coleoptera: Curculionidae) populations associated with Andean and coastal populations of Araucaria araucana in the La Araucanía Region, Chile
Luis Huala Jimeneza, Ramón Rebolledo Ranzb, Rubén Carrillo Lópezb, Mario Elguetac, Marco Paredes Honoratoa,,
a Universidad de La Frontera, Departamento de Ciencias básicas, Temuco, Chile
b Universidad de La Frontera, Departamento de Ciencias Agronomicas y Recursos Naturales, Temuco, Chile
c Museo de Historia Natural, Área de Entomología, Santiago, Chile
Recebido 25 Setembro 2017, Aceitaram 24 Dezembro 2017
Abstract

Calvertius tuberosus (Curculionidae) lives exclusively on Araucaria araucana trees (commonly known as pehuen) in southern Chile. In this study, morphometric and molecular genetic analyses of Andean and coastal populations of C. tuberosus were performed to evaluate evolutionary divergence associated with the discontinuity of the Araucaria forest between the coastal and Andean regions. Specimens of C. tuberosus were collected in Nahuelbuta National Park, Villa Las Araucarias, and Malalcahuello National Reserve and were classified and stored at the Animal Biotechnology Researching Laboratory (LINBA), University of La Frontera, Temuco, Chile. Thirteen morphometric parameters and the expression patterns of ISSR (inter-simple sequence repeat) markers were analyzed. Morphometric data revealed high phenotypic similarity between coastal populations. The genetic analysis revealed a high similarity between coastal populations (genetic identity, 93%), which were differentiated from the Andean population (genetic identity, 84%). This study contributes new genotypic and phenotypic data for the C. tuberosus populations in forest ecosystems of A. araucana, and clarifies the associations between these characteristics and the geographic distributions of populations.

Keywords
Araucaria forests, ISSR markers, Morphometry
Rev Bras Entomol 2018;62:119-24 - Vol. 62 Núm.2 DOI: 10.1016/j.rbe.2017.12.004