Revista Brasileira de Entomologia Revista Brasileira de Entomologia
Rev Bras Entomol 2015;59:240-50 - Vol. 59 Núm.3 DOI: 10.1016/j.rbe.2015.07.003
Medical and Veterinary Entomology
Morpho-histological characterization of immature of the bioindicator midge Chironomus sancticaroli Strixino and Strixino (Diptera, Chironomidae)
Vinicius Sobrinho Richardia, Maiara Vicentinia, Débora Rebechia, Luis Fernando Fávarob, Mário Antônio Navarro-Silvaa,,
a Laboratório de Entomologia Médica e Veterinária, Departamento de Zoologia, Universidade Federal do Paraná, Curitiba, PR, Brazil
b Laboratório de Reprodução e Comunidade de Peixes, Departamento de Biologia Celular, Universidade Federal do Paraná, Curitiba, PR, Brazil
Recebido 12 Fevereiro 2015, Aceitaram 18 Abril 2015
Abstract

Chironomidae immature are used as bioindicators of sediment quality in aquatic ecosystems and ecotoxicological assays. Histological descriptions for this family are outdated and limited and there are no studies with Neotropical species. The aim of this study was to describe the tissue architecture of several organs of the larva of Chironomus sancticaroli. For the description of the histological pattern, the larvae were fixed in Duboscq solution for insects at 56°C, followed by routine histologic processing, infiltration in paraffin, and the sections were stained with Hematoxylin–Eosin. After examining the slides, the tube digestive, salivary gland, excretory, nervous, endocrine, circulatory, and integumentary systems and fat body were histologically characterized. The histology allows evaluation of cell morphology, and for being not expensive and easily accessible can be routinely used in biomonitoring. In addition, is a useful tool in ecotoxicological assays and allow to evaluate biomarkers at tissue and cell levels.

Keywords
Ecotoxicology, Histological biomarker, Sediment
Rev Bras Entomol 2015;59:240-50 - Vol. 59 Núm.3 DOI: 10.1016/j.rbe.2015.07.003