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Vol. 61. Núm. 3.Julho - Setembro 2017
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Vol. 61. Núm. 3.Julho - Setembro 2017
Páginas 203-270
Short Communication
DOI: 10.1016/j.rbe.2017.05.004
Genetic divergence of a newly documented population of the cecidogenous micromoth Eugnosta azapaensis Vargas & Moreira (Lepidoptera: Tortricidae) in the Atacama Desert of northern Chile
Scott Escobar-Suáreza, Wilson Huanca-Mamanib, Héctor A. Vargasa,
Autor para correspondência
a Universidad de Tarapacá, Facultad de Ciencias Agronómicas, Departamento de Recursos Ambientales, Arica, Chile
b Universidad de Tarapacá, Facultad de Ciencias Agronómicas, Departamento de Producción Agrícola, Arica, Chile
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Tabelas (1)
Table 1. Nucleotide variation among haplotypes of the DNA barcode fragment (658bp) of the cytochrome c oxidase subunit I (COI) gene of Eugnosta azapaensis collected in the Azapa and Chaca valleys, Arica Province, northern Chile.

Eugnosta azapaensis Vargas and Moreira, 2015 (Lepidoptera: Tortricidae) is a micromoth native to the Atacama Desert whose larvae induce fusiform galls in shoots of Baccharis salicifolia (Ruiz & Pav.) Pers. (Asteraceae). The presence of this cecidogenous tortricid was previously recorded only from the type locality, the Azapa Valley, Arica Province, northern Chile. However, fusiform galls on shoots of B. salicifolia were recently found in Chaca, another coastal valley of the Atacama Desert. The adults obtained from these galls were preliminarily identified as E. azapaensis based on morphology. Subsequently, to assess an additional source of evidence for the taxonomic identification of E. azapaensis in this new locality, sequences of the DNA barcode fragment of the cytochrome oxidase subunit I mitochondrial gene from the two localities were analyzed. Four haplotypes were detected, two restricted to Azapa and two restricted to Chaca. The genetic divergence (K2P) between haplotypes of each locality was 0.2–0.8%, while it was 1.1–1.4% between haplotypes of different localities, and 8.7–13.5% between the Chilean haplotypes and other species of Eugnosta Hübner, 1825. In addition, all the sequences of Azapa and Chaca were clustered in a well-supported group in a Maximum Likelihood (ML) analysis. Accordingly, divergence and ML analyses support the morphological identification of E. azapaensis in the Chaca Valley. Furthermore, although preliminary, the analyses suggest that the genetic variation of the populations of this insect could be geographically structured, a pattern that must be assessed in further studies.

Baccharis salicifolia
DNA barcodes
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Tortricidae is one of the more diverse families of micromoths with more than 10,000 species described worldwide, with the highest level of species diversity in the Neotropical region (Regier et al., 2012). In a recent revision, Razowski and Pelz (2010) recorded more than 80 species from Chile, mostly based on the study of specimens from the central and south-central areas of this country. In contrast, the species from the arid environments of northern Chile have been scarcely collected and studied (Clarke, 1987; Bobadilla and Vargas, 2015). Larval feeding habits are variable in this family of micromoths. The larvae of many species are leaf-tiers and leaf-rollers; others bore into twigs, feed on reproductive organs (seeds, flower parts or fruits), or are gall inducers (Haghani et al., 2014; Razowski and Giliomee, 2014; Brown and Nishida, 2003). The gall-inducing habit has been described for several genera, mostly belonging to the tribes Cochylini, Grapholitini and Eucosmini (Brown and Nishida, 2007).

Eugnosta Hübner, 1825 (Tortricinae: Cochylini) is a worldwide genus with more than 90 species described, about 40 of which are from the New World (Gilligan et al., 2014). Natural history has been described for a few species of Eugnosta, and in the New World all are gall inducers in Asteraceae (Comstock, 1939, 1940; Goeden and Ricker, 1981; Vargas et al., 2015). Eugnosta azapaensis Vargas and Moreira, 2015 is the only representative of the genus currently recorded in Chile. Its larvae induce fusiform galls on shoots of the shrub Baccharis salicifolia (Asteraceae) in the Atacama Desert (Vargas et al., 2015). This cecidogenous micromoth was previously known only from the type locality, the Azapa Valley, Arica Province. However, fusiform galls were recently detected on shoots of B. salicifolia in the Chaca Valley, about 30km south the type locality (Fig. 1). The adults obtained from these galls were identified as E. azapaensis based on morphology of the male and female genitalia.

Fig. 1.

The study area in South America (left) and the sampling sites (right) of Eugnosta azapaensis in the Atacama Desert of northern Chile. The type locality Azapa Valley (black circle) and the newly documented locality Chaca Valley (black triangle).

The Atacama Desert is the most arid desert in the world (Clarke, 2006), where the coastal valleys of the northernmost part of Chile are recognized as valuable reservoirs of biodiversity for plants (Luebert and Pliscoff, 2006), birds (Estades et al., 2007), mammals (Ossa et al., 2016) and insects (Vargas and Parra, 2009; Méndez-Abarca et al., 2012). Accordingly, it is important to record the taxonomic composition accurately at a local scale, especially in little studied groups such as micromoths.

The analysis of different sources of evidence is important to reach sound taxonomic identifications (Dayrat, 2005; Schlick-Steiner et al., 2010). This approach is especially important when morphologically similar species are involved (Taft et al., 2016; Warren and Grishin, 2017), as is the case in many groups of micromoths (Landry and Hebert, 2013; Huemer et al., 2014; Kirichenko et al., 2015; Luz et al., 2015; Pereira et al., 2017). It has been shown that the analysis of the DNA barcode fragment (sensu Hebert et al., 2003) of the cytochrome oxidase subunit I is a useful complement to morphology for identifying small organisms, including species of the family Tortricidae (Jaeger et al., 2013; Brown et al., 2014; Gilligan et al., 2016; Corley and Ferreira, 2017; Razowski et al., 2017). Accordingly, DNA barcode sequences were analyzed to assess the taxonomic status of the newly discovered population of Eugnosta in the Chaca Valley.


Fusiform galls on shoots of B. salicifolia were collected in the Azapa (18°31′12″S, 70°10′41″W) and Chaca (18°48′56″S, 70°09′07″W) valleys between May and August 2016. The collected galls were placed in plastic bags and brought to the laboratory and dissected to extract the cecidogenous larvae, which were kept in 95% ethanol at −20°C for DNA extraction.

DNA extraction and sequencing

Genomic DNA was extracted from larvae following the procedures described in Huanca-Mamani et al. (2015) and subsequently was sent to Macrogen Inc. (South Korea) for amplification by polymerase chain reaction (PCR) and sequencing with the primers LCO-1490 and HCO-2198 (Folmer et al., 1994). The amplification program was: 5min at 94°C, 35 cycles of 30s at 94°C, 30s at 47°C, 1min at 72°C, and a final elongation step of 10min at 72°C.

Sequence analysis

According to the procedures described by Hall (2013), the software MEGA6 (Tamura et al., 2013) was used to perform the sequence alignment by the ClustalW method, to estimate the sequence divergence by the Kimura 2-Parameters (K2P) method and to perform a Maximum Likelihood (ML) analysis. Besides the Chilean sequences reported here, the following DNA barcode sequences (658bp) of Eugnosta with species identification were downloaded from BOLD (Ratnasingham and Hebert, 2007) for the ML analysis: E. beevorana (Comstock, 1940) (LNAUU4001-15), E. brownana Metzler and Forbes, 2012 (LNAUU4004-15), E. busckana (Comstock, 1939) (LOCB682-06), E. erigeronana (Riley, 1881) (LILLA210-11), Eugnosta sp. (LNAUU3996-15), E. percnoptila (Meyrick, 1933) (AFTOR133-12), E. sartana (Hübner, 1833) (LOFLA761-06). In addition, Aethes argentilimitana (Robinson, 1869) (BBLPA769-10) was included in the analysis, as Aethes Billberg, 1820 is close to Eugnosta (Regier et al., 2012). Previous to the ML analysis, the GTR+G was selected as the best model to describe the substitution pattern following the Bayesian information criterion. The statistical support of the nodes was assessed by 1000 bootstrap replicates. The genealogy of the haplotypes was inferred by the Median-Joining (MJ) method (Bandelt et al., 1999) in the software Network (Fluxus Technology Ltd.).

Eleven DNA barcode sequences (658bp) were obtained; four from Azapa and seven from Chaca (GenBank accessions MF000408–MF000418), all of which were characterized by 30.6% (A), 39.1% (T), 15.4% (C), 14.8% (G).

Genetic divergence

Eleven variable sites were found in the alignment of all the sequences of Azapa and Chaca valleys, with eight transitions and three transversions (Table 1). When the sequences of the different localities were aligned separately five variable sites were found in Azapa, while just one variable site was found in Chaca. The mutations determined the presence of four haplotypes, two (H1, H2) restricted to Azapa, and two (H3, H4) found only in Chaca. H1 and H2 were represented by two individuals each, H3 by six individuals, and H4 by one (Table 1). The divergence (K2P) was 0.8% between haplotypes of Azapa and 0.2% between those of Chaca. The divergence between haplotypes of different localities was 1.1–1.4%. Accordingly, the haplotype network shows a lower number of mutations between haplotypes of the same (1–5) than of different (7–9) localities (Fig. 2). The divergence between haplotypes of Chaca and Azapa with other species of Eugnosta included in the ML analysis was 8.7–13.5%.

Table 1.

Nucleotide variation among haplotypes of the DNA barcode fragment (658bp) of the cytochrome c oxidase subunit I (COI) gene of Eugnosta azapaensis collected in the Azapa and Chaca valleys, Arica Province, northern Chile.

  Variable sitesan  Locality 
  92  139  274  286  302  316  364  370  374  473  625     
Substitutionb  Ts  Tv  Ts  Tv  Ts  Ts  Ts  Tv  Ts  Ts  Ts     
H1  Azapa 
H2  –  –  –  –  –  –  Azapa 
H3  –  –  –  –  Chaca 
H4  –  –  –  Chaca 

“–” indicates nucleotide identity to the H1 haplotype.


Ts, transition; Tv, transversion.

Fig. 2.

Median joining network of the haplotypes of the DNA barcode fragment (658bp) of the cytochrome c oxidase subunit I (COI) gene of Eugnosta azapaensis from Azapa (white) and Chaca (black) valleys, Atacama Desert of northern Chile. H1, H2, H3, H4 haplotypes; circles proportional to the frequency of the respective haplotype; numbers between circles indicates variable sites; gray triangle a median vector.

DNA barcode divergence above 2% (K2P) is generally suggestive of different species of Lepidoptera (Hausmann et al., 2011). However, cases of higher intraspecific divergence have been reported, mostly involving widely distributed species (Wiemers and Fiedler, 2007; Hausmann and Huemer, 2011). In addition, cases of interspecific DNA barcode distances less than 2% also have been described, mostly dealing with morphologically close species (Kirichenko et al., 2015). Regardless of these exceptional cases, the divergence found between haplotypes of the Azapa and Chaca valleys (1.1–1.4%) appears to be at the intraspecific level. This suggestion is reinforced by the fact that the lowest divergence found between the other species of Eugnosta analyzed was 2.6% (E. busckana and E. beevorana).

ML analysis

The alignment for the ML analysis included nineteen DNA barcode sequences of 658bp length with 166 variable sites, 108 of which were parsimony informative. The eleven Atacama Desert sequences (Azapa and Chaca) were clustered with high support (Fig. 3), suggesting that all these belong to the same species (E. azapaensis). In addition, the sequences of Azapa were clustered with high support as a subgroup into the E. azapaensis clade (Fig. 3), suggesting that the genetic variation is associated with geographic distribution, although a similar scenario was not found for the sequences of Chaca.

Fig. 3.

Maximum likelihood tree of the sequences of the DNA barcode fragment (658bp) of the cytochrome c oxidase subunit I (COI) gene of Eugnosta azapaensis (gray area) from Azapa and Chaca valleys, Atacama Desert of northern Chile. Bootstrap supports higher than 70% are shown above branches.

The relationships of E. azapaensis with congenerics were not resolved in the analysis. However, this was expected since only a few species of Eugnosta are represented in BOLD, most of which are Nearctic. The Neotropical E. argentinae (Razowski, 1967) described from Argentina and E. ochrolemma (Razowski, 1986) from Mexico are morphologically similar to E. azapensis (Vargas et al., 2015), but DNA barcode sequences of these species are unknown. In addition, the gall shape of E. azapaensis is similar to that of the Nearctic E. busckana and E. beevorana. Interestingly, these two Nearctic species were clustered with high support in the ML tree, although they were not found to be close to E. azapaensis. Evidently, additional morphological (adult and immature stages) and molecular (mitochondrial and nuclear) data of other Neotropical species are necessary to understand the evolutionary relationships of E. azapaensis.

Further remarks

This is the first report of E. azapaensis outside the type locality. Galls of E. azapaensis were searched for on shoots of the only other species of Baccharis represented in the study area (B. scandens) without success. In addition, surveys for galls on B. salicifolia were carried out on the western slopes of the Andes of the Parinacota Province, at about 3000m elevation, also without success. Thus E. azapaensis appears to be a host-specialist with a geographic range restricted to the low elevation environments of the coastal valleys of the Atacama Desert. This geographic pattern has been described for different insect groups (Porter, 1985; Howden, 2008). Additional coastal valleys in which the host plant is present must be surveyed in order to better understand the geographic range of E. azapaensis along this extensive desert area.

The only DNA barcode sequence previously available for E. azapaensis was the analyzed in the original description of the species (GenBank accession KM023733.1; 622bp) which matches 100% with one of the haplotypes of Azapa (H2), while H1, H3 and H4 are here reported for the first time. The variation found in this study suggests that the DNA barcode fragment is a valuable tool to assess the taxonomic status of eventual new populations of Eugnosta of the Atacama Desert. Analysis of sequences of mitochondrial DNA is useful to explore geographic patterns of genetic variation, at least at a preliminary stage (Harper et al., 2008; Maita-Maita et al., 2015; Velasco-Cuervo et al., 2016; Maia et al., 2016). In this regard, an interesting finding is that each haplotype is restricted to a specific locality. This spatial segregation of the genetic variation suggests that the arid conditions of the area separating the valleys could be an effective barrier for the dispersal of adults of E. azapaensis, enhancing the genetic differentiation of the local populations of each valley. However, due to the low density of galls in the field, only a few specimens were collected and analyzed in this study. Thus further sampling and analyses of additional molecular markers (Valade et al., 2009; Seraphim et al., 2016) would be needed to verify this pattern of geographic structure of the genetic variation in the driest desert of the world.

Conflicts of interest

The authors declare no conflicts of interest.


The authors thank John W. Brown, Lucas A. Kaminski and two anonymous reviewers for valuable comments that substantially improved the final version of the manuscript, to Paula Escobar for providing Fig. 1 and Lafayette Eaton for checking the English. The study was supported by Project 9715-16 from Universidad de Tarapacá.

[Bandelt et al., 1999]
H.J. Bandelt,P. Forest,A. Röhl
Median-Joining Networks for inferring intraspecific phylogenies
Mol. Biol. Evol., 16 (1999), pp. 37-48
[Bobadilla and Vargas, 2015]
D. Bobadilla,H.A. Vargas
First record of Cydia tonosticha (Meyrick) (Tortricidae) from Chile and a new host plant
J. Lepid. Soc., 69 (2015), pp. 331-333
[Brown and Nishida, 2003]
J.W. Brown,K. Nishida
First record of larval endophagy in Euliini (Tortricidae): a new species of Seticosta from Costa Rica
J. Lepid. Soc., 57 (2003), pp. 113-120
[Brown and Nishida, 2007]
J.W. Brown,K. Nishida
A new gall-inducing tortricid (Lepidoptera: Tortricidae: Olethreutinae) on lima bean (Phaseolus lunatus; Fabaceae) from Costa Rica
Proc. Entomol. Soc. Wash., 109 (2007), pp. 265-276
[Brown et al., 2014]
J.W. Brown,D.H. Janzen,W. Hallwachs,R. Zahiri,M. Hajibabaei,P.D.N. Hebert
Cracking complex taxonomy of Costa Rican moths: Anacrusis Zeller (Lepidoptera: Tortricidae: Tortricinae)
J. Lepid. Soc., 68 (2014), pp. 248-263
[Clarke, 1987]
J.F.G. Clarke
Two new Cryptophlebia Walsingham from Chile (Lepidoptera: Tortricidae)
Acta Entomol. Chilena, 14 (1987), pp. 7-12
[Clarke, 2006]
J.D.A. Clarke
Antiquity of aridity in the Chilean Atacama Desert
Geomorphology, 73 (2006), pp. 101-114
[Corley and Ferreira, 2017]
M.F.V. Corley,S. Ferreira
DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae)
Zootaxa, 4221 (2017), pp. 594-600
[Dayrat, 2005]
B. Dayrat
Towards integrative taxonomy
Biol. J. Linn. Soc., 85 (2005), pp. 407-415
[Estades et al., 2007]
C.F. Estades,J. Aguirre,M.A.H. Escobar,J.A. Tomasevic,M.A. Vukasovic,C. Tala
Conservation status of the Chilean Woodstar Eulidia yarrellii
Bird Conserv. Int., 17 (2007), pp. 163-175
[Folmer et al., 1994]
O. Folmer,M. Black,W. Hoeh,R. Lutz,R. Vrijenhoek
DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates
Mol. Mar. Biol. Biotechnol., 3 (1994), pp. 294-299
[Gilligan et al., 2014]
T.M. Gilligan,J. Baixeras,J.W. Brown,K.R. Tuck
T@RTS: Online World Catalogue of the Tortricidae (Ver. 3.0)
Available from:
[Gilligan et al., 2016]
T.M. Gilligan,P. Huemer,B. Wiesmair
Different continents, same species? Resolving the taxonomy of some Holarctic Ancylis Hübner (Lepidoptera: Tortricidae)
Zootaxa, 4178 (2016), pp. 347-370
[Goeden and Ricker, 1981]
R.D. Goeden,D.W. Ricker
Life history of the gall-forming moth, Carolella beevorana Comstock, on the ragweed, Ambrosia dumosa (Gray) Payne in southern California (Lepidoptera: Cochylidae)
Pan-Pac. Entomol., 57 (1981), pp. 402-410
[Haghani et al., 2014]
A.F. Haghani,B. Yaghoubi,F. Majidi-Shilsar,N. Davatghar,L. Aarvik
The biology of Gynnidomorpha permixtana (Lepidoptera, Tortricidae) on Sagittaria trifolia L. (Alismataceae) in paddy fields in Iran
Nota Lepidopterol., 37 (2014), pp. 113-121
[Hall, 2013]
B.G. Hall
Building phylogenetic trees for molecular data with MEGA
Mol. Biol. Evol., 30 (2013), pp. 1229-1235
[Harper et al., 2008]
G.L. Harper,N. Maclean,D. Goulson
Molecular evidence for a recent founder event in UK of the Adonis blue butterfly (Polyommatus bellargus)
J. Insect Conserv., 12 (2008), pp. 147-153
[Hausmann and Huemer, 2011]
A. Hausmann,P. Huemer
Taxonomic decision as a compromise: Acasis appensata (Eversmann, 1832) in Central Italy—a case of conflicting evidence between DNA barcode and morphology (Lepidoptera: Geometridae)
Zootaxa, 3070 (2011), pp. 60-68
[Hausmann et al., 2011]
A. Hausmann,G. Haszprunar,P.D.N. Hebert
DNA barcoding the geometrid fauna of Bavaria (Lepidoptera): successes, surprises, and questions
[Hebert et al., 2003]
P.D.N. Hebert,A. Cywinska,S.L. Ball,J.R. deWaard
Biological identification through DNA barcode
Proc. R. Soc. B, 270 (2003), pp. 313-321
[Howden, 2008]
A.T. Howden
The species of Pandeleteius Schoenherr of coastal Chile and Peru (Coleoptera, Curculionidae)
Zootaxa, 1773 (2008), pp. 55-62
[Huanca-Mamani et al., 2015]
W. Huanca-Mamani,D. Rivera-Cabello,J. Maita-Maita
A simple, fast, and inexpensive CTAB-PVP-Silica based method for genomic DNA isolation from single, small insect larvae and pupae
Gen. Mol. Res., 14 (2015), pp. 7990-8000
[Huemer et al., 2014]
P. Huemer,O. Karsholt,M. Mutanen
DNA barcoding as a screening tool for cryptic diversity: an example from Caryocolum, with description of a new species (Lepidoptera, Gelechiidae)
ZooKeys, 404 (2014), pp. 91-111
[Jaeger et al., 2013]
C.M. Jaeger,J.J. Dombroskie,F.A.H. Sperling
Delimitation of Phaneta tarandana (Möschler 1874) and P. montanana (Walsingham 1884) (Tortricidae: Olethreutinae) in western Canada using morphology and DNA
J. Lepid. Soc., 67 (2013), pp. 253-262
[Kirichenko et al., 2015]
N. Kirichenko,P. Huemer,H. Deutsch,P. Triberti,R. Rougerie,C. Lopez-Vaamonde
Integrative taxonomy reveals a new species of Callisto (Lepidoptera, Gracillariidae) in the Alps
ZooKeys, 473 (2015), pp. 157-176
[Landry and Hebert, 2013]
J.-F. Landry,P.D.N. Hebert
Plutella australiana (Lepidoptera, Plutellidae), an overlooked diamondback moth revealed by DNA barcodes
ZooKeys, 327 (2013), pp. 43-63
[Luebert and Pliscoff, 2006]
F. Luebert,P. Pliscoff
Sinopsis bioclimática y vegetacional de Chile
Editorial Universitaria, (2006)
[Luz et al., 2015]
F.A. Luz,G.L. Gonçalves,G.R.P. Moreira,V.O. Becker
Description, molecular phylogeny, and natural history of a new kleptoparasitic species of gelechiid moth (Lepidoptera) associated with Melastomataceae galls in Brazil
J. Nat. Hist., 49 (2015), pp. 1849-1875
[Maia et al., 2016]
A.V.P. Maia,C. Almeida,K.R. Santoro,J.L.A. Melo,J.V. Oliveira,R.N.C. Guedes,C.A. Badji
High-level phylogeographic structuring of Neoleucinodes elegantalis Guenee (Lepidoptera, Crambridae) in Brazil: an important tomato pest
Rev. Bras. Entomol., 60 (2016), pp. 206-210
[Maita-Maita et al., 2015]
J. Maita-Maita,W. Huanca-Mamani,H.A. Vargas
First remarks on genetic variation of the little known leaf miner Angelabella tecomae Vargas & Parra (Gracillariidae) in the Atacama Desert of northern Chile
J. Lepid. Soc., 69 (2015), pp. 192-196
[Méndez-Abarca et al., 2012]
F. Méndez-Abarca,E.A. Mundaca,H.A. Vargas
First remarks on the nesting biology of Hypodynerus andeus (Packard) (Hymenoptera, Vespidae, Eumeninae) in the Azapa valley, northern Chile
Rev. Bras. Entomol., 56 (2012), pp. 240-243
[Ossa et al., 2016]
G. Ossa,K. Vilchez,P. Valladares
New record of the rare Long-Snouted Bat, Platalina genovensium Thomas, 1928 (Chiroptera, Phyllostomidae), in the Azapa valley, northern Chile
Check List, 12 (2016), pp. 1850
[Pereira et al., 2017]
C.M. Pereira,D.S. Silva,G.L. Goncçalves,H.A. Vargas,G.R.P. Moreira
A new species of Leurocephala Davis & Mc Kay (Lepidoptera, Gracillariidae) from the Azapa Valley, northern Chilean Atacama Desert, with notes on life-history
Rev. Bras. Entomol., 61 (2017), pp. 6-15
[Porter, 1985]
C.C. Porter
Trachysphyrus and the new genus Aeliopotes in the Coastal Desert of Peru and north Chile (Hymenoptera: Ichneumonidae)
Psyche, 92 (1985), pp. 513-545
[Ratnasingham and Hebert, 2007]
S. Ratnasingham,P.D.N. Hebert
BOLD: the barcode of life data system (
Mol. Ecol. Notes, 7 (2007), pp. 355-367
[Razowski and Pelz, 2010]
J. Razowski,V. Pelz
Tortricidae from Chile (Lepidoptera; Tortricidae)
SHILAP Rev. Lepidopt., 38 (2010), pp. 5-55
[Razowski and Giliomee, 2014]
J. Razowski,J.H. Giliomee
A new Afrotropical Chlidanotini species (Lepidoptera: Tortricidae) inhabiting galls of White Milkwood, Sideroxylon inerme, in South Africa
Afr. Entomol., 22 (2014), pp. 796-800
[Razowski et al., 2017]
J. Razowski,V. Pelz,S. Tarcz
Uncovering the hidden biodiversity of natural history collections: Insights from DNA barcoding and morphological characters of the Neotropical genus Orthocomotis Dognin (Lepidoptera: Tortricidae)
Zootaxa, 4250 (2017), pp. 541-559
[Regier et al., 2012]
J.C. Regier,J.W. Brown,C. Mitter,J. Baixeras,S. Cho,M.P. Cummings,A. Zwick
A molecular phylogeny for the leaf-roller moths (Lepidoptera: Tortricidae) and its implications for classification and life history evolution
PLoS ONE, (2012), pp. e35574
[Seraphim et al., 2016]
N. Seraphim,M.A. Barreto,G.S.S. Almeida,A.P. Esperanço,R.F. Monteiro,A.P. Souza,A.V.L. Freitas,K.L. Silva-Brandão
Genetic diversity of Parides ascanius (Lepidoptera: Papilionidae: Troidini): implications for the conservation of Brazil's most iconic endangered invertebrate species
Conserv. Genet., 17 (2016), pp. 533-546
[Schlick-Steiner et al., 2010]
B.C. Schlick-Steiner,F.M. Steiner,B. Seifert,C. Stauffer,E. Christian,R.H. Crozier
Integrative taxonomy: a multisource approach to exploring biodiversity
Annu. Rev. Entomol., 55 (2010), pp. 421-438
[Tamura et al., 2013]
K. Tamura,G. Stecher,D. Peterson,A. Filipski,S. Kumar
MEGA6: molecular evolutionary genetics analysis version 6.0
Mol. Biol. Evol., 30 (2013), pp. 2725-2729
[Taft et al., 2016]
W.H. Taft,A.I. Cognato,P.A. Opler
Phylogenetic analysis supports the recognition of Albuna beutenmulleri Skinner as a species distinct from A. pyramidalis Walker (Lepidoptera: Sesiidae)
J. Lepid. Soc., 70 (2016), pp. 211-217
[Valade et al., 2009]
R. Valade,M. Kenis,A. Hernandez-Lopez,S. Augustin,N. Mari-Mena,E. Magnoux,R. Rougerie,F. Lakatos,A. Roques,C. Lopez-Vaamonde
Mitochondrial and microsatellite DNA markers reveal a Balkan origin for the highly invasive horse-chestnut leaf miner Cameraria ohridella (Lepidoptera, Gracillariidae)
[Vargas and Parra, 2009]
H.A. Vargas,L.E. Parra
Prospección de lepidópteros antófagos asociados a Acacia macracantha Willd. (Fabaceae) en el norte de Chile
Rev. Bras. Entomol., 53 (2009), pp. 291-293
[Vargas et al., 2015]
H.A. Vargas,P. Pollo,D. Basilio,G.L. Gonçalves,G.R.P. Moreira
A new cecidogenous species of Eugnosta Hübner (Lepidoptera: Tortricidae) associated with Baccharis salicifolia (Asteraceae) in the northern Chilean Atacama Desert: life-history description and phylogenetic inferences
Zootaxa, 3920 (2015), pp. 265-280
[Velasco-Cuervo et al., 2016]
S.M. Velasco-Cuervo,L.L. Espinosa,D.N. Duque-Gamboa,M.F. Castillo-Cárdenas,L.M. Hernández,Y.C. Guzmán,M.R. Manzano,N. Toro-Perea
Barcoding, population structure, and demographic history of Prodiplosis longifila associated with the Andes
Entomol. Exp. Appl., 158 (2016), pp. 217-227
[Warren and Grishin, 2017]
A.D. Warren,N.V. Grishin
A new species of Oxynetra from Mexico (Hesperiidae, Pyrginae, Pyrrhopygini)
ZooKeys, 667 (2017), pp. 155-164
[Wiemers and Fiedler, 2007]
M. Wiemers,K. Fiedler
Does the DNA barcoding gap exist?—a case study in blue butterflies (Lepidoptera: Lycaenidae)
Copyright © 2017. Sociedade Brasileira de Entomologia
Revista Brasileira de Entomologia

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